Transparent, Nanoporous, and Transferable Membrane‐Based Cell–Cell Paracrine Signaling Assay | Semantic Scholar (2024)

A cellular layer-by-layer (cLbL) coculture platform using biodegradable, nanothin, highly porous (BNTHP) membranes better mimicked the in vivo 3D microenvironment and promoted cellular cross-talks between cocultured cells which occurred in nanoscale, resu...

Results showed that important nano-related parameters such as the diffusion, direct and indirect toxic effects of ZnO nanoparticles can be studied by combining this endoepithelial bilayer tissue model and traditional monolayer culture setting.

The co‐culture membrane developed in this study may provide an effective tool for recapitulating endothelial basem*nt membranes with a controllable endothelial barrier function.

The in vivo mimicking direct cardiac reprogramming and prevascularization technique can enhance the efficiency of cell sheets in clinical applications and provide new opportunities to prevent heart failure following MI.

Porosity of the PPE films resulted in increased efficiency of fluorescence quenching toward TNT vapors, which previously required very thin films for sensing applications, and constitutes a promising strategy for enhancing the efficiency of chemosensors and in more general applications requiring fine-tuned polymer-gas interactions.

A cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming is introduced.

The PAR film-based lubricant-infusedAgNPs-incorporated surfaces demonstrate outstanding antibacterial effects toward both waterborne and airborne Escherichia coli, suggesting a new door for development of an effective multimodal anti-biofouling surface.

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Cell phenotypical quantification results show that the cell spreading area per cell and optical density are useful parameters in distinguishing metastatic cancer cells from normal or fibrocystic disease cells on these LbL films.

The study identifies a nanostructured surface that retains stem-cell phenotype and maintains stem- cell growth over eight weeks, and implicates a role for small RNAs in repressing key cell signalling and metabolomic pathways, demonstrating the potential of surfaces as non-invasive tools with which to address the stem cell niche.

It was found that metastatic cancer cellAdhesion is affected by nanoparticle density on a surface, and ∼140 particles per 400 μm(2) (∼1.7 μm spacing between AuNPs) is optimal for effective metastatic cell adhesion.

It is reported that stacking and destacking layers of paper impregnated with suspensions of cells in extracellular matrix hydrogel makes it possible to control oxygen and nutrient gradients in 3D and to analyze molecular and genetic responses.

Several biochemical pathways that are important for the interaction between stromal and cancer cells with respect to energy metabolism, redox balance, cell survival and drug resistance are described.

It is revealed that integrin clustering and such adhesion induced by RGD ligands is dependent on the local order of ligand arrangement on a substrate when the global average ligand spacing is larger than 70 nm; i.e., cell adhesion is "turned off" byRGD nanopattern order and "turned on" by the RGD nanop attern disorder if operating at this range of interligand spacing.

A cell motility assay that allows competitive recruitment of a given cell population simultaneously by gradients of multiple cytotactic cues, observable under real-time imaging is developed.

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